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Biacore surface plasmon resonance spr technology
Surface Plasmon Resonance Spr Technology, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/surface plasmon resonance spr technology/product/Biacore
Average 86 stars, based on 1 article reviews

surface plasmon resonance spr technology - by Bioz Stars, 2026-05

86/100 stars

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( A ) Schematic demonstration of label-free EV capture bioassay using C1 sensor chips of Biacore TM SPR technology. Created with BioRender.com (not drawn to scale). SPR sensorgram representing ( B ) activation of C1 sensor chip followed by RAM immobilization and blocking steps and ( C ) mouse monoclonal antibody capture followed by EV binding and regeneration step. Reference refers to sample without addition of antibodies. Asterisk indicates time point at which amount of bound EVs was measured.

Journal: Biosensors

Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles

doi: 10.3390/bios16030174

Figure Lengend Snippet: ( A ) Schematic demonstration of label-free EV capture bioassay using C1 sensor chips of Biacore TM SPR technology. Created with BioRender.com (not drawn to scale). SPR sensorgram representing ( B ) activation of C1 sensor chip followed by RAM immobilization and blocking steps and ( C ) mouse monoclonal antibody capture followed by EV binding and regeneration step. Reference refers to sample without addition of antibodies. Asterisk indicates time point at which amount of bound EVs was measured.

Article Snippet: This study leverages surface plasmon resonance (SPR) Biacore TM technology to unveil the diagnostic potential of detecting CXCR4 on extracellular vesicles (EVs).

Techniques: Bioassay, Activation Assay, Blocking Assay, Binding Assay

Establishing a standardized SPR label-free bioassay for EV detection using rEVs. ( A ) The calibration curves using a series of rEV concentrations and anti-CD9, anti-CD63, anti-CD81 and anti-CXCR4 antibodies. The RUs were plotted as a function of the rEV concentrations (particles/mL). Simple linear regression was fitted using GraphPad prism software version 10.6. The LOD was calculated using (3.3*σ)/S = LOD, with σ being the standard deviation of the curve and S the slope of the curve. The error bars represent standard deviations (n = 3). ( B ) Stability and repeatability of the chip with different mouse antibodies. The capture levels are stable for at least 100 cycles. ( C ) Binding response from the three measurements of rEVs at a concentration of 1 × 10 9 particles/mL, illustrating interday precision.

Journal: Biosensors

Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles

doi: 10.3390/bios16030174

Figure Lengend Snippet: Establishing a standardized SPR label-free bioassay for EV detection using rEVs. ( A ) The calibration curves using a series of rEV concentrations and anti-CD9, anti-CD63, anti-CD81 and anti-CXCR4 antibodies. The RUs were plotted as a function of the rEV concentrations (particles/mL). Simple linear regression was fitted using GraphPad prism software version 10.6. The LOD was calculated using (3.3*σ)/S = LOD, with σ being the standard deviation of the curve and S the slope of the curve. The error bars represent standard deviations (n = 3). ( B ) Stability and repeatability of the chip with different mouse antibodies. The capture levels are stable for at least 100 cycles. ( C ) Binding response from the three measurements of rEVs at a concentration of 1 × 10 9 particles/mL, illustrating interday precision.

Article Snippet: This study leverages surface plasmon resonance (SPR) Biacore TM technology to unveil the diagnostic potential of detecting CXCR4 on extracellular vesicles (EVs).

Techniques: Bioassay, Software, Standard Deviation, Binding Assay, Concentration Assay

$( A – H ) SPR detection of tetraspanins CD9, CD63, CD81 and CXCR4 on purified EVs (SEC fraction 5). EVs were injected over C1 immunosensor functionalized with anti-mouse antibodies onto which anti-CD9, anti-CD63, anti-CD81 and anti-CXCR4 antibodies were captured (as depicted in ). Binding levels (RU) were normalized based on particle number, as explained in . Error bars represent standard deviations (n = 3). ( I ) Correlation of FCM data on cells and SPR data on EVs. FCM fold change data on cells is correlated with normalized data and is shown as logarithmic curve using Pearson’s correlation analysis, with r = 0.7960 ( p = 0.032) and 95% confidence interval (0.11–0.97).$

Journal: Biosensors

Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles

doi: 10.3390/bios16030174

Figure Lengend Snippet: ( A – H ) SPR detection of tetraspanins CD9, CD63, CD81 and CXCR4 on purified EVs (SEC fraction 5). EVs were injected over C1 immunosensor functionalized with anti-mouse antibodies onto which anti-CD9, anti-CD63, anti-CD81 and anti-CXCR4 antibodies were captured (as depicted in ). Binding levels (RU) were normalized based on particle number, as explained in . Error bars represent standard deviations (n = 3). ( I ) Correlation of FCM data on cells and SPR data on EVs. FCM fold change data on cells is correlated with normalized data and is shown as logarithmic curve using Pearson’s correlation analysis, with r = 0.7960 ( p = 0.032) and 95% confidence interval (0.11–0.97).

Article Snippet: This study leverages surface plasmon resonance (SPR) Biacore TM technology to unveil the diagnostic potential of detecting CXCR4 on extracellular vesicles (EVs).

Techniques: Purification, Injection, Binding Assay

Detection of EVs in crude mixture using Biacore TM SPR technology. Normalized binding levels of HEK293 and HEK293.CXCR4 EVs spiked in DMEM cell medium supplemented with 10% ED-FBS when using anti- CD9, anti-CD63, anti-CD81 or anti-CXCR4 as capture antibodies. DMEM cell medium supplemented with 10% ED-FBS was used as control. Binding levels (RU) were normalized based on particle number, as explained in . Error bars represent standard deviations (n = 3). Statistical difference between groups was assessed by 1-way ANOVA with Tukey’s multiple comparison test (alfa = 0.05) (*: p = 0.0109, ***: p = 0.0004 and ****: p < 0.0001).

Journal: Biosensors

Article Title: Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles

doi: 10.3390/bios16030174

Figure Lengend Snippet: Detection of EVs in crude mixture using Biacore TM SPR technology. Normalized binding levels of HEK293 and HEK293.CXCR4 EVs spiked in DMEM cell medium supplemented with 10% ED-FBS when using anti- CD9, anti-CD63, anti-CD81 or anti-CXCR4 as capture antibodies. DMEM cell medium supplemented with 10% ED-FBS was used as control. Binding levels (RU) were normalized based on particle number, as explained in . Error bars represent standard deviations (n = 3). Statistical difference between groups was assessed by 1-way ANOVA with Tukey’s multiple comparison test (alfa = 0.05) (*: p = 0.0109, ***: p = 0.0004 and ****: p < 0.0001).

Article Snippet: This study leverages surface plasmon resonance (SPR) Biacore TM technology to unveil the diagnostic potential of detecting CXCR4 on extracellular vesicles (EVs).

Techniques: Binding Assay, Control, Comparison

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